Measurement of Sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay.
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چکیده
منابع مشابه
Measurement of Sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay.
Lectin induced in the hemolymph of Sarcophaga peregrina (flesh fly) larvae on injury of the body wall or on pupation was studied further by radioimmunoassay, focusing on the interaction between the lectin and hemocytes. It was found that the amount of lectin on the surface of hemocytes prepared from injured larvae increased with time after injury of the body wall. Radioiodinated lectin could bi...
متن کاملPurification of lectin induced in the hemolymph of Sarcophaga peregrina larvae on injury.
A lectin was purified from the hemolymph of Sarcophaga peregrina larvae, obtained after injury of their body wall. This lectin agglutinated sheep red blood cells markedly and the hemagglutinating activity was inhibited by galactose and lactose. The active lectin was found to have a molecular weight of 190,000 and to consist of four alpha subunits and two beta subunits, with molecular weights of...
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Pricking the body wall of Sarcophaga peregrina (flesh fly) larvae with a needle activated the immune system of this insect and induced various immune molecules, including antibacterial proteins, in the hemolymph. In this review, I summarize and discuss the functions of these immune molecules, with particular emphasis on the dual roles of some of these molecules in defense and development.
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A sensitive, specific and precise procedure for the measurement of serum gentamicin by radioimmunoassay is presented. The method is rapid, convenient, and highly reliable for this very important measurement. Studies designed to evaluate the validity and reproducibility of the assay are presented and discussed.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)32899-0